Articular scanning and external counting in experimental synovitis in the guinea-pig.
نویسندگان
چکیده
Acute synovitis was induced in one elbow joint of 300 to 400 g. guinea-pigs by the intra-articular injection of the polyene antibiotic Nystatin (10,000 i.u. in 0 1 ml. physiological saline) (Weissmann, Pras, and Rosenberg, 1967). At 24-hr intervals for up to 7 days the intensity of the synovitis was evaluated clinically and by scanning and external counting techniques and compared with the non-injected elbow. Control studies were performed to ensure that the two elbow joints in untreated animals were similar and that the intra-articular injection of the same volume of physiological saline (0 1 ml.) did not alter the scanning or external counting characteristics of the joint. Radiographs taken after the injection of radio-opaque dye into nine elbow joints confirmed the reliability of the intra-articular injection technique. It was not found necessary to shave the area around the elbow joints. The guinea-pigs were anaesthetized by the intraperitoneal injection of sodium pentobarbitone and fixed to a board in a standard geometric pattern using rubber bands. Articular scanning and external counting studies were conducted with a Picker Magna-scanner with a 19-hole 3' focusing colimator after intravenous injection of radioiodinated (131) human serum albumin (RISA) in a dose of 10 to 20 uc./kg. The pulse height analyser was set to accommodate the 364 Kv peak of the 1131 spectrum. Shielding of the trunk of the animal was not found to be necessary. Scintillographic studies were unsatisfactory as the small areas involved allowed insufficient definition. Although RISA is fully mixed in the peripheral blood within 10 to 15 minutes of the intravenous injection, the RISA was injected 24 hrs before the first scanning and external counting assessment to allow it to reach equilibrium with the interstitial and synovial fluids. It was found that a single injection of RISA (20 jFc./kg.) provided adequate residual radioactivity for scanning and external counting procedures to be carried out for up to 7 days. An attempt was made to determine the percentage of radioactivity in the area of the joint contributed by RISA circulating in the hypervascular tissue compared to the percentage contributed by RISA in the interstitial and synovial fluids. Synovitis was induced and RISA (10 ,uc.) injected in three animals on Day 0 and the synovitis was assessed by external counting on Day 4. Then a further injection of RISA (lOutc.) was given intravenously and a re-assessment of the synovitis made after an interval of 20 minutes. In an additional three animals, RISA (10 jc.) was injected on Day 0, and synovitis was induced on Day 3 and assessed on Day 4 before and after a second injection of RISA (10 ,uc.).
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ورودعنوان ژورنال:
- Annals of the rheumatic diseases
دوره 30 2 شماره
صفحات -
تاریخ انتشار 1971